What is the recommended serology sampling schedule after exposure when monitoring a previously vaccinated animal?

• A. Day 0: draw a sample; Day 5-7: second serum sample
• B. Day 0; Day 3
• C. Day 1; Day 2
• D. Day 7; Day 14

Answer: (A)

When a previously vaccinated animal is exposed, you’re looking for evidence of an anamnestic antibody response rather than a simple, baseline level. The best way to capture that response is to take a serum sample at the time of exposure (baseline) and then a second sample about five to seven days later. This five-to-seven-day window is when memory B cells are most likely to boost antibody production, so a rise in titer between the two samples helps confirm recent exposure despite existing vaccine antibodies. Sampling too early (within a day or two) often shows little or no change, while waiting longer (like day seven to day fourteen) can delay confirmation and intervention. The key is paired samples that demonstrate an increase in antibodies from the initial baseline, indicating exposure or infection in the vaccinated animal.